The Basics of Crispr-Pro: Knockout Mice

Some people may think that genetic knockouts are created more often using the method that’s most commonly described in all of the introductory biology videos and textbooks; and while using stem cells does work, crispr has actually become the standard method used to generate these animals as its faster turnaround time, lower cost, and higher targeting efficiency has led to its widespread use in the industry.

Using the SHANK3 gene as an example, this video shows you how scientists preform crispr-pro knockouts in mice as well as other animals.


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Cyagen is the worlds leading provider of genetically modified mice and rats. We have produced over 50,000 projects to date. We also specialize in embryonic stem cells and the special media used in this video for research purposes.

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[madymeldrum]

Can you explain why/how CRISPR/Cas9 would be used in conjunction with Cre/LoxP recombination? It seems like both can create knockout mice, so is it not redundant then to use them together?

[lissakaye]

I have only worked with an autistic mouse model once and they were nuts. Absolutely adorable, but very difficult to handle. Very interesting video, thanks.

[mgold]

At 4:55 speaker says: "as there are mulitple cells in the zygote" But this is incorrect! The zygote is the fertilized egg at the one cell stage. Then it is a 2 cell stage embryo, 4 cell stage embryo, 8 cell stage embryo and then morula, blastula, blastocyst. What the speaker is after is that the gene knock out in the genome may not occur at the one cell stage i.e. in the zygote but at a later stage like in the 2 cell stage or 4 cell stage embryo creating a mosaic. Right?

[gingerlee]

Last year I had mice crewing the wires inside my gas fireplace. I had mice inside the condo walls and I had them LIVING inside my fridge. I could not even imagine why mice were made at all and hated them so much that I didn't really feel bad when they got stuck on glue boards. And now here they are, mice helping cure all disease.

[tomg.]

Can this be applied to my wife's Myasthenia Gravis for a cure?

[salmazara]

I would like to know what makes you so much jealous of