The Principle of Pulsed Field Gel Electrophoresis (PFGE)

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This video provides the best explanation about Pulsed Field Electrophoresis, it helps you to fully understand the principle and the technique.
Watch also Agarose Gel Electrophoresis:

Biomedical and Biological Sciences

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I must say this is the best video for pulse field gel electrophoresis on YouTube. Not a single indian educator explained this technique as beautifully as you did. Thanks

Sumit_parihar

Thank you very much for this video! You're an amazing teacher.

liliancruz

now you got me reminiscing. i used a PFGE set up when i worked in cancer research years ago. The hope was to find a set up that could be scaled up to workout if patient A, B or C needed the *full dose* of cancer treatment or hopefully slightly less.

i have black hair if i waned to dye it red i’d have to use one hell of a lot of red dye (probably have to bleach it first then dye it red). Someone with blonde hair would not need the extra step & would need less dye. So if thats true about hair colour, we are all different amounts then it surely must be true about DNA.

i was trying to explain all this to a friend so glad to come across this video.

Thou not sure you quite described how fiddly the bloody thing was, especially in summer. So i have lovingly made my little tiny squares with human dna. i then zap the poor little squares of gel with x-rays, causing the double strand breaks. Then the little squares of jelly get their own back as using a biology fume hood with your hands up inside & then in that position you had to get each jelly square balanced on the tip of a scapel, sometimes trim it CAREFULLY and place it into the well.

Sounds easy until you think (especially in summer) you are trying to fit two bits of jelly together, neatly & accurately You have to be cardful put it in with no mushing it in or breaking the plug. The more you mush it up you are creating more double strand breaks. There were times lol. i just 😶😶😶. Txs for the work you are doing here i might nerd out & have a look around.

Is couple of videos showing the lab equipment, my baby, but you certainly explained it best.

Thanks for a trip down memory lane.

appppppplecrumble

Dear lady i cannot thank you enough. ❤❤❤❤❤

YusufNusretKurkcuoglu

Other questions are about the parameter setting:
1. I think a larger angle should be better for resolution since the route can be longer. I do not understand why you said the smaller angle is better.
2. You mentioned that the time and voltage should be the same for turning the right and left direction. But as the DNA elongates, its moving speed will also change. So although you use the same voltage and time, it may still cannot be straight. How to deal with that?

yijingwang

Great video! Really easy to follow. However, I have a question concerning the switching time parameter: When I want to perform this technique, the system asks me to input two values (for example: initial time 10 sec; final time 30 sec). What exactly mean those two values??

NicoLamannaR

The best explanation.. ver easy to understand

bhargaviminchu

simplified and throughly explained...
great work..
Thank You

mukuldev

Thank you for your video. But I am confused why do not we just extract and elongate DNA samples outside and put them into the wells? So we do not need to use several currents to elongate them.

yijingwang

Excellent presentation and well-explained! Thank you!

debjyotipaul

Brilliant brilliant explanation thank youuu

noufalssab

Excellent explanation, lucid too. thank you

reginaamabanu

how does PFGE change when doing it in a capillary? because i think that in capillary, there is only one direction possible for the electrical field right?

isaacyoung