Measuring noisy images using binary masks in ImageJ

A quick imageJ tutorial. Using binary masks to extract objects and measure intensity in noisy images.

[vetdrpradip]

Thanks a lot Craig very important videos with valid and relevant content

[stevenmendoza]

Thank you Craig! Saved my life! doing image processing on fibers and found this really helpful thanks!

[brycecarr]

Great Videos Craig! This information is also suer helpful for Earth Scientists! Please keep up the good work. Loving Greetings from Germany.

[hamid]

thank you so much for this great video. please do share more .. really great stuff :)

[muratseker]

thank you for the video. I appreciate it and would like to see more image J videos

[ThanhNguyen-heyt]

Hi Craig! Thank you so much for this great video. I was able to learn a lot from it. Not sure if you will see this comment of mine but thought it would worth the try. I have to count neutrophil cells in zebrafish larval's hindbrain using ImageJ software. struggle at the moment as the neutrophil cells do not have clear boundaries e.g like colonies but appear in patches instead. I just wonder if I can get some help which I greatly appreciate :)

[TheClaretDoctor]

Thanks for all your videos Craig, really great. Any tips on following a similar process on 32-bit float images? Perfoming an image calculation of the 32-bit original AND 8-bit mask leads to odd results (not identical to converting the original to 8-bit). Would like to keep my raw 32-bit scale rather than converting to a 0-255 integer scale

[dumisilelumkwana]

Hi Craig, thank you for this video. Just wanted to make sure if I want to calculate the intensities, I would the use image 3?

[azarmassah]

Thanks again for making these great videos. Can we use the dilated Binary image directly to analyze particles? actually, I did not get what is the purpose of the last step (image calculator)?

[FrozenGravy]

Thanks for the video! Does the process of binary masking (changing pixel values throughout this process) still allow for measuring intensity, for example, since the end result seems to have matching pixel values, or is measuring only suitable for the original raw image?

[ojgfhuebsrnvn]

Thank you. I need a bit different thing but this buide helped me a lot. However i have a question, can i save picture without background? What i need is outline nanograins and outline function/ find edges function/ or this method do with some error (sometimes it outline 5-10 grains as 1 grain). SO i want to save picture of edges without background and use it as second layer in, for example GIMP so i would be able to separate those grains manually

[logicallybio]

I need to measure the intensity for my experiments. I am a bit confused. Should i "multiply" or "add" my original image with the threshold image.

can anyone please let me know??

Thanks

[Ibrahim]

Dear Craig i have 2 images i want to change thise pictures to good quality one. Can you help me ?