Genetic Variant Detection in gene by Melting Analysis | Protocol Preview

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis - a 2 minute Preview of the Experimental Protocol

Tadej Pajič, Tanja Belčič Mikič, Helena Podgornik, Jurka Klun, Sandra Šućurović, Samo Zver, Matjaz Sever
University Medical Centre Ljubljana, Department of Hematology; University Medical Centre Ljubljana, Clinical Institute for Genomic Medicine; University of Ljubljana, Faculty of Pharmacy;

High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.

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