10 - Bradford Assay

In this video, we use Bradford assay for protein quantification.

[SewayPL]

This is a gorgeous learning resource thank you so much. I wish there were more demonstrations like this of lab protocols online.

[NasarKhanU]

can we check westernblot prepared samples with bradford? prepared sample mean samples with lemille buffer

[imranali-fmsx]

How to prepare sample for maximum adsorption of BSA? Can you explain in detail

[rawantej]

can you please explain how to prepare x and how to calculate the initial concentration of x in your exemple because i didnot realy get the calculation from the dilution

[KIEUMANHUYTMU]

Dear Mister
I am quite confuse at 13:24 . Does it mean value = 0.488 must be in between 0.43 and 0. 52 of the y axis?
Thank you

[fatimahamel]

I want a written rapport for this technique (materils, method, objectif and

[nitikaaversatileworld]

How much Bradford reagent it's not audible

[georgenikolaras]

Shouldnt the curve be starting from the beginning of the two axes (point 0, 0)? Because if you have no absorbance you cant have concentration. And also, isnt the standard curve a version of Lambert-Beer's law? If so, we cant have equation of y=ax + b formation, we will have the formation y=ax. And the thing i cannot understand is why we do the standard curve. Cant we just use the bradford reagent and measure the complex absorbance and after that, using Lambert-Beer's law we find the protein's concentration? Please correct me if im wrong.

[mehmoodulhassan]

sir I want it in written form can you provide it

[freshd]

Great video! If you ever plan on recording a new version, you might add the standard deviation and error calculations to the Excel part of the video.

[mohi]

Really great. But may I ask what is X?