DNA Libraries & Generating cDNA

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In this video, I discuss the generation of DNA libraries from complementary DNA (cDNA).
  1. sahyouniron
  2. dna
  3. khan
  4. khan academy
  5. mcat
  6. prep
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This video misrepresents DNA libraries:

- It conflates "DNA libraries" with "gene sequence databases". A DNA library is not a computerized database; it is a physical collection of actual DNA segments, generally stored in recombinant plasmids taken up by e.g. a population of E coli or yeast cells.

- It ignores the distinction between genome libraries (containing all an organism's DNA) and cDNA libraries (containing only expressed DNA), the latter being why proteins and mRNA are relevant at all.

- It claims that you can use the AA sequence to derive the mRNA sequence; but that's impossible, due to the redundancy of the genetic code. It also implies that the mRNA sequence is known; but if it was known, we could just derive the DNA sequence by base-pairing rules, we wouldn't need reverse transcriptase or sequencing at all (if the goal was to put it in a database). In fact, the mRNA is itself physically isolated without knowing its full sequence, then used to create the cDNA.

davidbahry