How are DNA Libraries Made for NGS? #shorts

NGS libraries get made by cutting the DNA into short pieces of a specific size. This cutting gets done using high-frequency sound waves or enzymes. Then sequences of DNA called adapters get added to each end of a DNA fragment. These adapters contain the information needed for sequencing. They also include an index to identify the sample. Finally, any non-bound adapters get removed, and the library is complete. Depending on the application, there can be a PCR step to increase the library amount. A successful library will be of the correct size. It will also be of a high enough concentration for sequencing.⁠

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#ngs #sequencing #shorts

[loveteaching]

Best most concise explanation ever, thank you!

[muhammed]

Best explanations ever! Thank you for producing them. I've watched all your videos, is there a resource to access more?

[Yhusi]

Great explanation. Thanks, May I know what about the library in RNA Seq?

[SmileyFaceLolz]

Thank you, how does the index help identify the sample? I always wonder how do you know how to put it all back in order? Thank you so much!!

[MukherjeeKeya]

Why final Library sizes always should be higher than WG size?