filmov
tv
📈 4 PARAMETER LOGISTIC CURVE fitting for unknown concentration calculation | Adwoa Biotech
Показать описание
4-parameter logistic curve fitting - when and why to use it!
The classic way that unknown concentrations of proteins or biological assays are determined is to first create a standard curve and determine the signal absorbance (Absorbance or fluorescence or luminescence) - in a suitable instrument versus its concentration.
The line of best fit for bioassays such as ELISA and dose response experiments is not always linear because bioassays are only linear in a very specific concentration magnitude. Dose response experiment assessment should use a sigmoidal curve fit for certain interactions. A sigmoidal shape occurs when the x-axis values of rectangular hyperbola is set to a logarithmic scale. Sigmoidal curve fits are analysed with four parameter logistic (4PL) regression models where the Minimum concentration, Maximum concentration, Inflection point (IC50/EC50) and Hill coefficient is analysed. Dose response is often used interchangeably with a related term Concentration Effect Curve. However, the latter is performed outside of living systems such as in cell lines. Excel does not allow such fits as default, so in this video I will show an online tool for 4PL curve fits.
.
.
.
.
.
.
.
.
.
.
.
.
This video is about 4 parameter logistic curve, 4 parameter model, 4 parameter sigmoidal curve fit, 4pl curve fit, 4 parameter logistic curve formula, ELISA data analysis, non linear regression, linear regression, elisa data, elisa data analysis.
.
.
.
// ADWOA
I'm Adwoa (Adwoa Biotech), a Biotechnology graduate. I've worked in medical research for years and want to be useful to people new to the lab life. This channel takes you through some of the techniques and concepts I've had to learn working as a Research Assistant to hopefully help others when they are new to the topic/technique.
0:14:10
📈 4 PARAMETER LOGISTIC CURVE fitting for unknown concentration calculation | Adwoa Biotech
0:07:54
ELISA Analysis in Excel with 4PL
0:03:48
Logistic Regression in 3 Minutes
0:09:25
CurveFit 4: Examples Using Excel and Trendline
0:04:21
4PL and 5PL Data Analysis in Excel with MyCurveFit Excel Add-In
0:14:22
Logistic Regression [Simply explained]
0:09:14
Logistic Regression - THE MATH YOU SHOULD KNOW!
0:20:25
Logistic regression : the basics - simply explained
1:26:36
Parameter estimation
0:06:24
How to fit non-linear equations in excel using solver
0:01:18
Path Planning using Four Parameter Logistic Curves
0:06:30
Item response theory using Stata: One-parameter logistic (1PL) models
0:19:52
7.2.4. Gradient Descent for Logistic Regression
0:17:15
Logistic Regression in R, Clearly Explained!!!!
0:06:09
ELISA Data Analysis - Tools for Performing 4PL and 5PL Fits
0:06:51
Understanding the Summary Output for a Logistic Regression in R
0:10:23
Logistic Regression Details Pt 2: Maximum Likelihood
0:04:15
The logit model explained in 3 minutes
0:04:07
How To Interpolate A Standard Curve In GraphPad Prism
0:04:40
Important tuning parameters for LogisticRegression
1:06:19
CS 480/680 - Lecture 4 - Logistic Regression
0:10:24
Estimating Parameters in Logistic Regression || Machine Learning ||
0:14:37
Competitive ELISA Tutorial 3: Analyzing Typical Competitive ELISA Data in Excel
0:04:46
logistic regression: parameters
Комментарии