Streaking for Single Colonies

Monoclonal liquid bacterial cultures should be started using single colonies. Watch this video to learn how to effectively streak and grow single colonies on plates.

For the full written Streaking for Single Colonies protocol, visit:
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0:00 Intro
1:14 Materials
1:39 Protocol
3:35 Incubation and Results
4:02 Key Points for Optimal Results

[doricenguemadje]

What do you think it is important to have individual colonies in a streak plate? Streak plate techniques for the isolation of pure culture video. Thank you.

[sofi]

Do we need to dip in bacteria culture for every single toothpick or just for the very first toothpick?

[doricenguemadje]

What do you think it is important to have individual colonies in a streak plate? Thank you

[dorfinhorg]

How important is sterile technique when working with antibiotic media? Ab should kill contaminants, right?

[lapetitebantam]

As far as I'm aware gloves should not be worn when working next to the flame