Making a Streak Plate

This video demonstrates the streak plate method for isolating a single colony of bacteria from a culture. Proper use of a bunsen burner, inoculation loop, and aseptic technique are shown. This activity is included in the laboratory textbook Biotechnology: A Laboratory Skills Course.

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[acmbendorf]

Very thorough, one comment when sterilizing the loop it is better to start at the hilt and proceed to the loop to reduce aerosolization of bacteria

[dbhbiochem]

When the narrator says rotate 45 degrees, this should be 90 degrees for a quarter of a turn. Other than that, great video

[Waterdreamer]

I am extremely curious who the narrator of these Basic Biotechnology Techniques is. I think I want to be him for Halloween? Can you ask him if that would be okay? He's had a great influence on me and the rest of my Evolution and Genetics High School class. He's my personal hero.

[virginiagarcia]

what would the reagents be in this streak plate method?

[yugioh]

what would happen if you break the surface of the agar? when I do streak plate I sometimes press the loop hard and it go inside the agar but not all the way..also after each time you flame the loop do you have to let it cool down before streaking it in the plate?

[yarrowthistle]

This will only work on a 100mm plate. It is too many passes for a 60mm one. I am really struggling to get pickable colonies with a 60mm...so little surface area!

[trivespaulus]

i think the video is okay its very helpful..

[professord]

The quadrants should also be clearly labeled in advance.

[jasontheandonly]

Wouldn’t the remaking heat in the tool kill the Bacteria?

[shrutimishra]

Please hindi midium ke student ke liye bhi hindi me video banaye😢😢😢

[easterbunny]

This vid was made when i was 5 bruh only seeing this for college hw 😂

[xELLIExDEEx]

Only problem I can see with this is that he's wearing gloves around a Busen burner, which is actually very dangerous. I'm assuming that those specific gloves aren't flammable but a lot of gloves in a laboratory are and so it would be a good idea to mention this so that students wouldn't repeat