11 - SDS-PAGE

In this video, we show how to set up a SDS-PAGE experiment and separate proteins by their molecular weights.

[CR-uurr]

Congratulations, I have never seen a tutorial as clear, detailed step by step and above all as complete as this one. Truly a lectio magistralis. Thank you, you are super.

[DEDSE]

Thnx this video change my life for better ❤🙌🏻

[alialmoussawi]

you need to add something between the stacking and seperating so that your gel will be leveled. You can use isopropanol or butanol or simple DW, during the 30 - 60 mins wait time. Read Schagger protocol. Many issues.

[okyanusya]

Thank you, finally i understand total procedure.

[EramFatima-xu]

Why did your gel lanes appear blue after running SDS PAGE? I mean when you dismounted the gel, the lanes looked blue and not much difference after staining?

[manpreetkour]

how to purify protein from adherent cells ?

[leticiadamianodantas]

how much marker do you put on the gel?

[NAZIMUDDIN-pxrm]

Honourable Sir./Maam. I feel that it need to add yours video speech sub-title.

[danieloulhint]

This video is good but the music background is so disturbing