Phage Display

Used for the Cambridge Science Festival to explain our Phage Display Technology

[carl-johanhorberg]

Thanks! Finally an explanation that puts it into a functional context.

[veraltol]

i have been in difficultes somme days to cleary understand this method. i was stressed but this video helped me a lot! Merci beaucoup aux auteurs;

[Biomeducated]

The fishing can be done in several ways, depending on the target of interest and how it can be recombinantly produced (or not). For instance, you can interrogate your library of phages for selection on solid phase (where the antigen is coated on a plate), or in solution (where the antigen is coupled to beads). If you're antigen is difficult to produce (for instance a GPCR), you could select in solution on recombinant CHO or HEK cells expressing the target (allowing proper folding of the target). Furthermore, selection strategies can be designed for diversity, or for affinity, all depending on the goal of the discovery strategy. Informative introductory video on Phage Display! Cheers

[danielgladish]

Wow! Great explanation and super interesting technique! Very high throughput

[belindashaw]

Would this be useful to other protein expression platforms, say in plants such as tobacco? And if you wanted to make a unique antibody that you didn't have the antigen for how could you go about that...? Thanks for the video was very helpful :)

[chrissun]

Very clear description. But I am confused how people know the gene can be expressed as an antibody inside the phage.

[amonoracheal]

Thank you for making it clear for me:)

[oidualclaudi]

Is this an alternative for the use of animals like rabbits and mice to produce antibodies?

[Biomeducated]

Excellent explanation and animation! It's also used to 'fish' for Nanobodies! If you don't know what Nanobodies are, I made an introductory video on my channel. Great video, Charlie Abbott!

[daoyuzhang]

This could be what was used to generate the 100x higher-binding-affinity ACE2 RBD in Covid-19. Such high binding affinity have never been found in nature for betacoronaviruses in general.

[paffnblunt]

from where do we take the genes at the beginning?

[sandybravo]

just wanted to know if we call it phage antibody or antibody phage

[ViskayaNuebler]

I think you helped me understand a paper i neededt to read