NCBI NOW, Lecture 2, The FASTQ File Format

Learn how to process fastq files, the most common raw data file in next generation sequencing (NGS), so that they are ready for alignment and subsequent variant calling. Dr. Sijung Yun provides a detailed description of the anatomy of these files, as well as how to filter them for quality, which is critical for almost all projects. The filtering tutorial is presented in the galaxy framework, an excellent environment for learning NGS.

[hyrunnisa]

i took 2 years of bioinformatics classes and never learned this. wtf...thank you for the in-depth lesson.

[InfiniteExplora_World]

Excellent explanation. Thank you so much, Sir.

[saty]

link that you provided is not working in 2020 !

[tzatzikosouvlaki]

does this apply to Ion Torrent Proton sequencing data as well ?

[BilalAhmed-ibyw]

The link is broken, not working anymore, Pls suggest.