Southern blotting

Southern blotting - This lecture explains about the southern blotting procedure and technique to identify specific DNA fragment from a mixture of DNA molecules separated by the Agarose gel electrophoresis. Southern blotting step by step explanation is provided to understand the procedure of the DNA separation and identification. Southern blotting application is also explained in this video.
Southern blotting consists of three different stages -
1. First is the isolation of DNA from the target cell.
2. Then the treatment of restriction digestion of the DNA to generate DNA fragments
3. Then the separation of the DNA fragments using Agarose gel electrophoresis
4. Transfer of the Agarose gel DNA bands to a nitrocellulose Membrane filter. This filter paper after the DNA transfer is known as southern blot.
5. Then the use of complementary DNA probe to bind with the target DNA that will indicate the presence of target sequence by radioactivity or colorize trick expression. Southern blotting was invented by EM Southern on 1975 at it was named after him to identify DNA using blotting technique.
For more information, log on to-
Get Shomu's Biology DVD set here-
Download the study materials here-
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-

We are social. Find us on different sites here-
Thank you for watching southern blotting lecture.

[nilutpaldas]

Sometimes I think why don't we get professor like you in university?
U r like Complete this line friends

[shamimahmed-pkug]

I'm a student of biotechnology. I found that ur 100time's better than my prof. Thank you sir. May god bless you.

[TheRealNG]

You are a machine my friend. I've watched a lot of videos about SB but a clear explanation of the principle of blotting was nowhere to find until I found your video. Thank you for helping me and you have my respect for what you do here. Sharing and explaining the information is an honorable thing to do. I am glad that there are such people as you.

[강아지-mj]

I LOVE your accent. I can easily understand because you point out all the important parts with accent. And also you know what kind of questions listeners would have and you answered all of them. It is like taking a private lesson. Thank you so much! From Korea :)

[aashu]

Thank you sooo much sir for your efforts .... i must say that you are the best biology teacher i ever have

[chayanikagogoi]

Sir your biology lectures has saved me in my graduation and now they are still a blessing for me in my master's degree.🥺..thankyou so much sir

[BTSARMY-sbsh]

SAYING "THANK U" WILL NEVER ACTUALLY THANK YOU SIR.
YOU HAVE MADE THIS TOPIC CRYSTAL CLEAR TO ME BY YOUR AMAZING TEACHING STYLE. U. ARE. THE. BEST. I NEEDED U. YOU HELPED ME FOR THE PREPARATION OF MY PRESENTATION. U ARE AN ABSOLUTE GREAT TEACHER.I CAN NEVER THANK U ENOUGH EVER!!!
LOTS OF LOVE & RESPECT FROM PAKISTAN 💜🌸, FEELING BEYOND LUCKY TO FIND YOUR CHANNEL.💜😇 YOUR NEW SUBSCRIBER 👉💜👈

[drbabaranwarkhan]

Very comprehensive.
I am a doctor and you have very well explained about all blotting methods.Thank you

[lauraprince]

Thank you for making this video. You've explained this way better than my prof!

[shereen]

I have an exam on Thursday on this and I never understood this process until I listened to you explain it. Thank you so so much for your help❤️

[misbahmalik]

This man is MASHALLAH :) I've just started watching his tutorials and getting information that I couldn't get during my B.S and M.S. No one can teach better than him. ALLAH bless u

[devonfernando]

No professors explained what southern blotting really is. So you are the best professor for me. Thank you 😊

Good luck sir,
From Sri Lanka 🇱🇰

[midnightdreamer]

it took more time to read d same thing from book but just some minutes r required to understand d same thing in more clear way from your lectures...the way u taught d thing and make us learnt is really awesome.

[ACHIEVEWN]

i have been watching your vids since many years now.. and you have really improved in ur explanations... ur older vids are nice as well.. but the newer ones are even better.. i prefer this format over the older s.blot video!

[xurshidbekumarov]

Thank you very very much. There is no such explanation in the youtube even in 2022. May God bless you.

[pallavikhele]

somus biology is one of the most interesting online class an I can able to get any topic easily in my mind I love this channel in the form of understanding level

[Gaeforhae]

Whenever i need to clear my doubts i type the topic name in youtube followed by shomu’s biology because literally nobody clears my concepts like he does, he is a blessing sent to us by gods 😭😭

[lonelygamerzwala]

Sir you are a savior of my life specially before exam . You teach every portion in a very simplified and lucid way . Always keep us helping like this. Thank you sir.

[shireenroy]

You are a life savior sir! I tried to understand this multiple times for 1and half month couldn't get but now I have got it clearly. U just saved me

[minaristic]

Great video! I have Southern Blotting as the subject for my biology presentation, and this is the only thing that helped me understand what Southern Blotting is. Thank you!