SEM of Embryo, Eggshell, and Fungal Cultures | Protocol Preview

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy - a 2 minute Preview of the Experimental Protocol

Jessica Gibbons, Poongodi Geetha-Loganathan
SUNY Oswego, Department of Biological Sciences;

Here, we present detailed processing protocols for imaging delicate tissue samples using scanning electron microscopy (SEM). Three different processing methods, namely, hexamethyl disilazana (HMDS) chemical drying, simple air drying, and critical point drying are described for preparing rigid eggshells, embryos at early developmental stages, and fungal cultures respectively.

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