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Prof Tom Brown - Artificial nucleic acid backbones for Synthetic Biology
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15 June 2023
Chemical ligation of DNA strands can be used to produce artificial DNA backbones which have potential for use in therapeutics and synthetic biology.1-6 I will discuss recent developments from our laboratory7, 8 and present data on the effects of various DNA backbone analogues on duplex stability. In addition, I will discuss results from next-generation sequencing analysis of modified DNA templates that can be read through and copied accurately by DNA polymerases. These results provide insights into the design of biocompatible backbone mimics that could be used in the assembly of large modified DNA constructs and used in various applications9 including Crispr gene editing.10 Preliminary work on the use of artificial DNA backbones in therapeutic oligonucleotides will also be presented.11
Chemical ligation of DNA strands can be used to produce artificial DNA backbones which have potential for use in therapeutics and synthetic biology.1-6 I will discuss recent developments from our laboratory7, 8 and present data on the effects of various DNA backbone analogues on duplex stability. In addition, I will discuss results from next-generation sequencing analysis of modified DNA templates that can be read through and copied accurately by DNA polymerases. These results provide insights into the design of biocompatible backbone mimics that could be used in the assembly of large modified DNA constructs and used in various applications9 including Crispr gene editing.10 Preliminary work on the use of artificial DNA backbones in therapeutic oligonucleotides will also be presented.11
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