Protein DNA Bioconjugate Protocol | Bioconjugation

In this video, we discuss a protocol to create a simple protein dna bioconjugate that can add DNA to a protein in a site-specific manner. This bioconjugation technique utilizes simple organic chemistry reactions with high yield.

Adding DNA to protein is useful for a variety of techniques including immuno PCR, studying protein-DNA interactions, Protein barcoding, etc.

A protein dna bioconjugate can be created using both site-specific methods as well as broader methods like reactions with lysines or cysteines on the protein. SIte-specific protein modification has the advantage that it won't reduce the protein's specific activity if done properly.

Steps covered in this video to site-specifically make a bioconjugate of Protein and DNA include:
- Introducing azides via site directed mutagenesis into the protein
- Adding a cyclooctyne onto DNA by using a PEG linker and NHS amine chemistry
- SPAAC or click chemistry to conjugate the DNA and the protein
- Using Gel electrophoresis for analysis of the conjugate and to determine stoichiometry

Want more protocols like this?

Keywords related to this method include:
dynamic biosensors,pure protein-dna conjugates,protein dna bioconjugate protocol,bioconjugation,protein dna interaction,dna protein synthesis,protein-dna synthesis,attach dna to protein,bioconjugation chemistry,bioconjugation techniques,bioconjugation antibody,conjugate protein and dna,protein bioconjugate,protein bioconjugation,protein dna coupling,protein fingerprinting technique,protein barcoding,scigine,dna barcoding protocol,dna barcoding technique

[scigine]

Why do you want to conjugate DNA and Protein? Are you barcoding? Comment below