TALENs - TALE NUCLEASES - GENE EDITING EXPLAINED!

This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.

This presentation describes the architecture of TALE nucleases, which are engineered proteins used for genome editing.

Zinc finger nucleases (ZFNs)
CRISPR adaptive immune systems
Using CRISPR/Cas9 for genome editing
CRISPR specificity
Precision editing
Homology directed repair
Prime editing

This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

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More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.

Publication List
10. Kim H, Kim JS.
A guide to genome engineering with programmable nucleases.
Nat Rev Genet. 2014 May;15(5):321-34.

12. Boch J, Bonas U. Xanthomonas
AvrBs3 family-type III effectors: discovery and function.
Annu Rev Phytopathol. 2010;48:419-36.

13. Bogdanove AJ, Schornack S, Lahaye T.
TAL effectors: finding plant genes for disease and defense.
Curr Opin Plant Biol. 2010 Aug;13(4):394-401.

14. Mak AN, Bradley P, Cernadas RA, Bogdanove AJ, Stoddard BL.
The crystal structure of TAL effector PthXo1 bound to its DNA target.
Science. 2012 Feb 10;335(6069):716-9.

15. Joung JK, Sander JD.
TALENs: a widely applicable technology for targeted genome editing.
Nat Rev Mol Cell Biol. 2013 Jan;14(1):49-55.

16. Mussolino C, Cathomen T.
TALE nucleases: tailored genome engineering made easy.
Curr Opin Biotechnol. 2012 Oct;23(5):644-50.

Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)

[qwertyhghgmhvmgh]

RVD stands for Repeat Variable Diresidue. The amino acids in the middle are called central TAL targeting domain.

[xavierwong]

Watched your video on ZFNs and CRISPR as well, has helped a heap with my assignment. Thanks you for the great videos 👍

[anirakbenadam]

Your explantions are very clear, I enjoyed your videos and learned a lot. Thank you very much!!!

[jackpertzel]

Extremely helpful making my assignments much easier Thank you!!

[stanleysalivan]

Great video; Shortly and very clearly explained. Thank you!

[brandonunger]

Really enjoying this series of videos
Thank You

[TheDRNA]

Great video, detailed yet short thank you

[mohammadhammad]

ITS REALLY A GOOD PRECISE AND HELPFUL VIDEO BE BLESSED

[caiorodriguescorrea]

Thank you for the very helpful video and good job on the channel!

[carissejadeangulo]

Extremely helpful in making my topic discussion for one of my plant breeding subjects. Thank you so much sir!

[karolinawasilewska]

Very clear and helpful presentation. Thanks you so much :))

[jamesraymond]

Excellent! I finally understand it. However, one question: at 3:40 where the TALENs are biding to the DNA, it seems that the TALENs are too bulky to fit close enough together to bind to successive nucleotides.

[kiara_l]

is this method susceptible to off target cleavage like with CRISPR enzymes?