Method of the Year 2011: Gene-editing nucleases - by Nature Video

Gene-editing nucleases can make targeted and precise changes to an organism's genome. This has opened up new possibilities for the study of gene function, as well as the treatment of disease. While gene-editing nucleases have been in use since the mid-1990s, in the form of zinc finger nucleases, the more recent discovery of TALENs (transcriptor-like effector nucleases) has created new interest. In this video, Nature Methods technology editor Monya Baker explains how gene-editing nucleases work and why they were chosen as Nature Methods 'Method of the Year' for 2011.

[mightbin]

ZFNS AND TALEN ARE STILL VERY SPECIFIC GENE EDITING TOOLS, BUT NOT EASY TO DESIGN,
IN COMPARISION, CRISPER ARE VERY EASY TO HANDLE

[caneycrew]

There is also proximity- the nucleases won't cut unless they form a heterodimer (2 parts coming together to form an active enzyme). Therefore, it takes both "arms" of the TALEN binding specifically to generate the specific cut.

[emilyb]

lol can't believe this was posted before CRISPR was discovered

[sarabpreetkaur]

zinc finger nickases are enzymes that just "nicks" one strand therefore tipping the DNA repair towards homology dependent repair and thus maximum specificity and lower toxicity.

[VeritYandMusiC]

Are the two parts that cut each strand connected or are they separate? How precise is the cut? Can it be engineerd to cut only in one place?

[eddaceccarelli]

it's a very very good explanation

[variasdiaz]

Es importante en el conocimiento del campo de la investigación genética

[MichaelPSchroeder]

@IQ20000Berta I do not entirely understand what you mean by structure, but I don't thnink there is a special reason. After all it should be an illustrative video.

[vinchino]

Zinc finger and TALENs are old news, CRISPR-CAS system is the future

[SurajKumar-bwoi]

CRISPR Cas-9 be like: Hold my scissors

[armoda]

lol at CRISPR being discovered the next year

[thianhnguyen]

Character In the video It's great, I like it a lot $$

[masoudy]

This is a sign for high school students: major in molecular biology
We will need people to store DNA data for 7 billion people after a few years

[MarkMoore-lg]

Williams John Williams Timothy Allen Matthew

[herpaderp]

I'm wondering where the hell she picked that up. Has she not heard it correctly enough times?!

[valarieapperson]

Hell yeah! UH students have tomorrow off!!!