Human iPSC-Derived Cardiomyocyte Networks For Recurrent AP Recordings

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings - a 2 minute Preview of the Experimental Protocol

Viviana Zlochiver, Stacie L. Kroboth, Christopher R. Beal, Jonathan A. Cook, Rosy Joshi-Mukherjee
Advocate Aurora Health Care, Aurora Research Institute; University of Wisconsin-Milwaukee, Department of Biomedical Engineering, College of Engineering and Applied Science; Johns Hopkins University, Department of Medicine-Cardiovascular, School of Medicine;

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

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