[MERL Seminar Series Fall 2023] Robust and Physics-informed machine learning for low light imaging

Kristina Monakhova, MIT, presented a talk in the MERL Seminar Series on November 28, 2023.

Abstract:
Imaging in low light settings is extremely challenging due to low photon counts, both in photography and in microscopy. In photography, imaging under low light, high gain settings often results in highly structured, non-Gaussian sensor noise that’s hard to characterize or denoise. In this talk, we address this by developing a GAN-tuned physics-based noise model to more accurately represent camera noise at the lowest light, and highest gain settings. Using this noise model, we train a video denoiser using synthetic data and demonstrate photorealistic videography at starlight (submillilux levels of illumination) for the first time.

For multiphoton microscopy, which is a form a scanning microscopy, there’s a trade-off between field of view, phototoxicity, acquisition time, and image quality, often resulting in noisy measurements. While deep learning-based methods have shown compelling denoising performance, can we trust these methods enough for critical scientific and medical applications? In the second part of this talk, I’ll introduce a learned, distribution-free uncertainty quantification technique that can both denoise and predict pixel-wise uncertainty to gauge how much we can trust our denoiser’s performance. Furthermore, we propose to leverage this learned, pixel-wise uncertainty to drive an adaptive acquisition technique that rescans only the most uncertain regions of a sample. With our sample and algorithm-informed adaptive acquisition, we demonstrate a 120X improvement in total scanning time and total light dose for multiphoton microscopy, while successfully recovering fine structures within the sample.