Micropipetting

Micropipettes are devices that are used to deliver small volumes of liquid; usually in the range of 0.5 to 1,000 microliters. Like other forms of laboratory instrumentation, the micropipette must be operated with precision so that your measurements can be reproduced consistently.

It's not hard; so don't be intimidated by it. But it does take practice. Your technique is very important. And since a micropipette delivers such small volumes, a minor operational flaw could make a huge negative impact on the accuracy of your measurements.

In this video, we'll demonstrate the correct technique for using a micropipette. We'll also examine some common micropipetting mistakes.

This video was filmed on location at Gaston College.

[pete]

Great. Managed to identify my mistakes i been committing for my 1st 2 lab sessions. Thanks for the invaluable techniques and steps!

[paessex]

Millipore, Sigma-Aldrich and other major manufacturers recommend tip pre-wetting.

[kurrizzle]

This was very helpful, thank you so much for making such a useful video publicly available!
Are there any more-advanced techniques or tips that could supplement what is covered in the video? Also, I really liked the production value of the video. It felt good to watch.

[mountcarmel]

Awesome demonstration; short video with lots of information.

[prasadvlogs]

I like your way of presentation and pronunciation thank you sir

[Khadija.Mohammed]

thank you it's too easy to understand your explanation

[AhmedGamalMedicine_gets_easy]

Thank you 
Amazing short and informative indeed :)

[mothrakaiju]

This is why I love science just picking up liquids

[techpriest]

Because there may still be liquid in the tip or on outside of tip and they could be different samples of same liquid

[olliestringer]

The issue I have, is that when pipetting using the correct technique in the video the demonstrator still has liquid in his pipette when he discards the tip. Surely if you're pipetting you should have expelled all the liquid by going to the second stop? And I've had PhD students say to me when pipetting, don't produce bubbles in the micro-plate (or w/e) but by going to the second stop you've expelled all liquid so air ensures nothing's left. How do I follow their instructions without pipetting wrong? :/

[duraiswamy]

how to prevent air bubble during mpn while transfering the water

[SurvivalSquirrel]

why is every tip thrown away when it is the same

[vuatrochoi]

I like it, these kind of video >.<  very nice  instruction video, it help me a lot >.<

[anonymus]

Hilarious. Minute 2:18. There's liquid left in the tip! Worst transfer ever!

[xhapinalicious]

200 & 1000 easy than other pipetteing