How to Set Up the PCR Reaction

This video is the second part of a 3-part Getting started with PCR series that shows how to set up and run a PCR experiment.

The success and failure of any PCR experiment is dependent on preparation. This particular video shows the different components that go into a PCR reaction, how each component contributes to the experiment, and lastly, when to set up a master mix.

[gowheregodgoes]

:( All I could think about, was the tip boxes are open the whole time.... Can't concentrate!

[corbinmerrill]

Would this be the step that can be considered creating dilutions. I am doing a independent project for my school and it ahs to do with PCR and Electrophoresis. I am stumped on the Quantification, standardization, and dilutions part. I did this project a year ago and I am using it again but I do not have all of my data I used when doing this and it is bit of a headache.

[Tepmodify]

Thank you ...good vedeo.
When is you protect contamination.

[lancelyde]

great video! I have a PCR lab tomorrow

[অধোরাআলো]

Very helpful video on PCR. Many many thanks for uploading this.

[coachjeremydraws]

Does that meant the template DNA was added to the tube?

[setanta]

stopped short on stating NUMBERS of cycles used 🤔🤔

[rimodotika]

why is she using glasse during manipulation ?

[VladimirGent]

awful, sorry... it is for Chinese students in university , not for DIY-Bio amateurs.