GCSE Biology - Genetic Engineering Insulin

A quick tutorial, showing how we use restriction enzymes to cut out a desired gene from one organism, and insert it into the plasmid of a bacterium. This allows massive production of the desired protein (in this case the hormone: insulin) in a relatively short amount of time.

[nhl]

Thankyou
Made it very easy for me...
Thanks keep going. :)

[chockpea]

Where does the gene come from for human insulin production? What human body part is being used when the gene is extracted? Thank you

[dani_ako]

Thx....really helped my revision for science

[mehrabhabib]

Sir, you haven't talked about the extra sticky ends which the restriction enzymes make; sticky ends are totally required when mixing DNA with the plasmid because complementary base pair needs to be form between them.

[enyaaquilina]

thanks for the awesome video, its very clearly explained :)

[penguinfancy]

What about Lysozyme and Ligase enzymes?

[weiweiweiwei]

Do you know where to find more details on the following process to the completion of using E. coli 12 to produce insulin? Where can I learn of the ideal Fermentation Media, Temperatures and so on? Thanks for a great intro video. Cheers

[ScatteredDreams]

hey, i know some people are sort of salty due to the fact that you missed some enzymes but this video did it for me. I need to explain to people how insulin is made & I don't really have to go into dept with those enzymes. Thanks!

[kashofficial]

ANYONE DOING LAST MINUTE REVISION?? xD

[badr]

I hate biology, it is so annoying. I'm failing biology :(