Rock the Clone with our molecular cloning solutions so you can clone loud and proud!

The molecular cloning workflow requires many steps to obtain the perfect clone for downstream applications. Striking the right note at every step in the cloning workflow—clone, transform, purify, and analyze—is key to successfully producing a recombinant clone quickly.

With all of our cloning solutions, it is time to rock your workflow and rock your clone.

Step 1 – Clone

Molecular cloning relies on recombinant DNA technologies to insert a DNA sequence of interest into a vector to generate a large number of copies of the recombinant vector. Cloning has traditionally required restriction enzymes and a DNA ligase to form a new recombinant vector; however, recent cloning advancements such as TOPO cloning, ligation independent cloning, and gene synthesis provide more efficient workflow solutions.

Step 2 – Transform

After the DNA fragment is cloned into a vector, competent bacteria are transformed with it to propagate sufficient quantities of the cloned DNA for downstream experiments. The choice of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications.

Step 3 – Purify

Once the bacterial cells are transformed with the vector, it is critical to recover plasmid DNA using plasmid purification products that are optimized to provide maximum yield, purity, and integrity.

Step 4 – Analyze

Choosing the right tools for your cloning analysis can significantly improve and accelerate results, enabling you to address downstream applications sooner. Explore our do-it-yourself reagents, isolation kits, and precast analysis options to find the right workflow solution for your cloning analysis needs.

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