Using Micro-CT Imaging for the Phenotyping and Analysis of Bone Architecture

Presented By:
Rob van 't Hof, BSc, MSc, PhD - Professor of Musculoskeletal Biology The Institute of Ageing & Chronic Disease University of Liverpool.

Speaker Biography:
Rob van 't Hof is professor of musculoskeletal biology at the University of Liverpool. His main interest is the regulation of bone turnover, especially in relation to age related bone loss and bone disease. He has extensive experience in using µCT for phenotyping transgenic animal models of bone disease. Using these techniques, he has investigated the role of different nitic oxide synthases in bone, developed a mouse model of Paget's disease of bone and developed a novel class of anti-inflammatory drugs that also inhibit bone resorption. He has more than 10 years' experience in bone phenotyping of transgenic mice, and during this time has developed approaches for high throughput CT analysis. To complement the µCT analysis he has also developed software for semi-automated dynamic bone histomorphometry.

Webinar:
Using Micro-CT Imaging for the Phenotyping and Analysis of Bone Architecture

Webinar Abstract:
Micro-computed tomography (micro-CT) offers significant advantages over other X-ray imaging techniques in the field of bone research. Its high-level X-ray absorption enables good contrast and the ability to observe bone in very fine detail, not only in bone density studies, but also those examining the actual architecture of the bone.

Seminar Overview

In this webinar, professor of musculoskeletal biology, Rob van 't Hof, will discuss the application of micro-CT for the analysis and phenotyping of bone. Most of the studies described will be ex vivo, but a few examples of in vivo micro-CT will also be given. Rob and team have used micro-CT to assess bone phenotype in transgenic mice, to test the efficacy of drugs targeting post-menopausal osteoporosis and to understand the bone condition Paget’s disease. For their studies, the team has used Bruker’s ex vivo SkyScan 1272 with automated sample loader as well as a SkyScan high resolution in vivo micro-CT.

What to Expect

Attendees will hear how micro-CT can be used to analyse mouse bone phenotypes and the bones’ response to osteoporosis drugs, as well as how to look in vivo at the bone changes that occur in mice with Paget’s disease over their lifespan. Automated analysis of the scans will also be discussed.

Earn PACE Credits:

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