What do bacteria look like under a light vs electron microscope?

Check out how our scientists prepare bacterial samples to look at them under light and electron microscopes. The reagents used and the protocols followed are different - check out what these are and what purpose do they serve. And finally see how bacteria looks like under a microscope.

Typo at 0.25: Take 0.01 ml (not 0.1ml) of overnight culture and add it to 1ml of fresh medium to dilute it 100 times.

[MICROWORLDofficial]

You forgot to specify that you used not just light microscopy, but the DIC method! This is very important, because bacterial cells will hardly be distinguishable under conventional light-field microscopy. The DIC method gives a certain dimension to the investigated objects (something like the oblique illumination method, but with greater contrast and resolution in details).

P.S. Not the best music for such videos, really.

[Antelocapra]

Why do you have 99% of the video taken up with the preparation (ie stuff we don't care about) and only a few seconds showing us the microscope images?

[helpinghands]

Cells dilution should be 10 times not 100 times. Correct me, if I am wrong.
(0.1ml culture from dense medium to 1ml of fresh medium).

[michaelanderson]

The atoms of living organisms cannot be seen (my opinion). However, the atoms in non living things can be seen (metal).

[sandrawilkinson]

Showing too much unnecessary footage. 😔