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Genetic transformation in the era of GENOME EDITING
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QAAFI Science Seminar
Presented by Professor Kan Wang, Director of the Center for Plant Transformation, Iowa State University
Description
TALENs and CRISPR/Cas9 are powerful genome editing systems that have been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species. Researchers at Iowa State University Crop Bioengineering Consortium have been engaged actively in the development of a public sector genome editing platform. Here an Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis is described. This system was evaluated for its mutagenesis frequency and inheritability using maize genes. When crossed with a wild type maize line, null segregants carrying only the desired mutant alleles but free of CRISPR reagent transgene could be generated in as early as T1 progeny.
QAAFI on Twitter: @QAAFI
Presented by Professor Kan Wang, Director of the Center for Plant Transformation, Iowa State University
Description
TALENs and CRISPR/Cas9 are powerful genome editing systems that have been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species. Researchers at Iowa State University Crop Bioengineering Consortium have been engaged actively in the development of a public sector genome editing platform. Here an Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis is described. This system was evaluated for its mutagenesis frequency and inheritability using maize genes. When crossed with a wild type maize line, null segregants carrying only the desired mutant alleles but free of CRISPR reagent transgene could be generated in as early as T1 progeny.
QAAFI on Twitter: @QAAFI
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