Cell Culture Training Video

[truecanuck]

For those who are not familiar with centrifugation, you may have to ignore the 1, 000 rpm recommendation in this video. Depending on the rotor size, that means you may be applying less or more gravitational force than you want to. Potentially, using 1, 000 rpm for your centrifuge setup will result in not collecting all or damaging cells in the process. It is more helpful to give parameters for centrifugation in # x g (meaning some number times gravity). x g is always equal no matter what centrifuge you use, whereas rpm is dependent on rotor size and is associated with a specific x g for that rotor size/centrifuge model.

[KJKP]

Dr. Clean adjusted her eye protection with her gloved hand. Since she earlier donned those goggles before gloving up and sanitizing... she just cross contaminated the outside of the container.

[MsMoniss]

Ethanol will not work if you wipe it off with dry cloth... the whole point of sanitizing with ethanol is to WET what you want to sanitise and LET IT DRY! minimum contact time with ethanol is 5 mins. the actual sanitation is taking place WHILE DRYING as ethanol supposed to dehydrate all of the moister from the bacteria

[HeavyWater]

Thanks for the video, I officially begin learning cell/tissue culture tomorrow at a laboratory at Hopkins for skin conversion/regeneration!

[ShakespeareCafe]

The weak link in aseptic technique is the glass pipette container. They are crammed in the metal box necessitating grabbing one while touching the others, then pulling the one out the possibility of tip contamination results. I use my finger tip to carefully push up just the pipette I will use and avoid touching the others.

[funny]

Very nice, and helpful. Very helpful comments that complete information. Toghether forms corect tehniques. Good.

[platynowa]

What is the point of a lab coat if you got your hood on it? Also, your lab coat sleeves are too short, so your bare skin comes out, this is why cuffed lab coats are much better, and it is easy to put the cuff in your glove. That shelf obstructs the airflow at the back of the BSC, it shouldn't be there, there is a lot of stuff that is not required for current work and has to be cleaned up in case of spillage. The handling of the bottles is also sacrilegious in both cases, you should avoid putting away the caps, you should put the pipette back in the packaging to not scatter stuff what is left in it around. I use 0.1 of a culture volume of trypsin, so for T25 it would be 0, 5 ml. Glass pipettes can be easily replaces with cheap pump nozzle that fit standard one-use pipette tips. 500 g is a recommended force for precipitating the cells. From earlier I assumed you use a medium containing serum, so unless you switch to serum free media that centrifugation step is completely unnecessary. FBS contains a very strong trypsin inhibitor. You also lift the bottles when not necessary, risking dropping them and spills, this can be comfortably done without all that lifting and putting away caps. You also touch your glasses when removing cells from the BSC - this is unacceptable. You are also not supposed to open anything that may contain living cells outside the BSC.

[nunitchagucci]

I love this video!!! This is so helpful and super funny. Thank you!!! 😊❤️❤️

[KHUSHALI]

There is one mistake. Never put the cap of bottle same side at which we fit it on bottle but put cap opposite side when we open it. (Media bottle)

[muhammetmemon]

minute 8:44 supernatant aspirated
minute 9:46 magically media appears in the test tube.
Sorry I missed the step in between. After aspirating the supernatant do we add fresh media? If so how much ml? Thanks.

[akalkhanpashteen]

Der kha wallah...nar pashteen bahdar pshteen...
Manzoor pashteen manzoor pashteen

[zed]

what is next step after the supernatant is aspirated? How was the cell pallet resuspended in media? 

[edisfchen]

Why there is are some classical music there, hahah I can’t focus on the lecture, cuz the music is too beautiful !

[Palalita]

Dr. Clean must learn to cover and uncover the tube with one hand to avoid waiting to use both hands to cover it... :/ and some other... many things...

[philiptunge]

The mistakes I noticed in this video is not working atthe centre of the hood as this is considered asceptic area due to lamina flow, please rectify that

[shareefsyed]

Lmaoo I wish my lab made videos like this

[waisx]

Thank you very much for sharing the information =)

[AbdulGaniganibhai]

dam mad, keeping hand out to put used pipette every time, kept unwanted material in bio safety cabinet, no mask, head cover.

[imagination]

What is the name of the piece of classical music playing in this video at 07:30?

[imrocknreeling]

I didn't understand where the hell that pallet gone? 🤔