Corrected Total Cell Fluorescence CTCF analysis using ImageJ Software

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Thank you for your straightforward explanation! This helps a lot.

midnightrose
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Thank you for the clear demonstration! When performing this analysis on a z-stack which type of z-projection would be appropriate? Sum of slices, average intensity etc...?

kaylafleming
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CAN WE DO THIS IF OUR IMAGE IS STAINED WITH HOESCHT ONLY, AND TAKEN ON DAPI CHANNEL, DO WE NEED TO SPLIT CHANNEL EVEN THEN?

asmaali