How to SEGMENT cells and COUNT the numbers and MEASURE their AREAS using ImageJ Software

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Hello ! thank you very much for your video, very useful and well explained. I wonder if it is possible to transfer this process to a macro to automate this process, I know very little of batch work but I have seen in some cases that it is done, would it be possible in this case ?

rubenvelez
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Just wondering why you didnt utilize dapi staining to segment nucleus and then propagate them to find

jamilaiqbal
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Applying this procedure, do you think there will be differences between cells from fresh apple tissues and oxidized apple tissues (i. e., minutes after cut)? Will the cell morphology, size or shape change? Furthermore, using the area or circularity of cells, will I be able to verify significant differences between both apple tissues states? Thanks for the video

andresalbertocorimayhuasil
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Hi, thanks for the video
I have a question, how we can count the cells if the color of the cells quite similar with the background and the cells have gradient color.
Thanks

yogiagustion
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In images like this: green is in cytoplasm AND nucleus. How do I quantify the green that is ONLY inside the nucleus without having neither the DAPI nor the green in the cytoplasm interfere with the signal?

FarahRSS
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Hi. Thanks for this great content. Can you make a video on cell segmentation using brightfield images

jamilaiqbal