Pluripotent Stem Cells Differentiation into Liver Cells | Protocol Preview

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells - a 2 minute Preview of the Experimental Protocol

Kyle M. Loh, Amrita Palaria, Lay Teng Ang
Stanford University School of Medicine, Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute; Stanford University School of Medicine, Department of Developmental Biology;

This protocol details a monolayer, serum-free method to efficiently generate hepatocyte-like cells from human pluripotent stem cells (hPSCs) in 18 days. This entails six steps as hPSCs sequentially differentiate into intermediate cell-types such as the primitive streak, definitive endoderm, posterior foregut and liver bud progenitors before forming hepatocyte-like cells.

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