New Mass Spectrometry Based Technologies for Biophysics and Structural Biology- by Prof. Matt Bush

Native-like ions are generated using electrospray ionization of proteins, nucleic acids, lipids, and other biological molecules in aqueous solutions. These gas-phase ions can retain noncovalent interactions that were present in the original solution, and consequently, native ion mobility (IM) mass spectrometry (MS) has great potential for answering many questions in biophysics and structural biology that have eluded condensed-phased strategies. I will report new technologies that my lab developed to probe the structures and interactions of proteins. I will then discuss how my lab has applied these technologies to answer questions related to ubiquitin-mediated protein degradation.