Lactate Dehydrogenase Activity Monitoring : Mapping Metabolism | Protocol Preview

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue - a 2 minute Preview of the Experimental Protocol

David Jelinek, Aimee Flores, Melanie Uebelhoer, Vincent Pasque, Kathrin Plath, M. Luisa Iruela-Arispe, Heather R. Christofk, William E. Lowry, Hilary A. Coller
UCLA, Department of Molecular, Cell and Developmental Biology; David Geffen School of Medicine, Department of Biological Chemistry; UCLA, Molecular Biology Institute Interdepartmental Program;

We describe a protocol for mapping the spatial distribution of enzymatic activity for enzymes that generate nicotinatmide adenine dinucleotide phosphate (NAD(P)H) + H+ directly in tissue samples.

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