mRNA Transcripts Stability Identification by Plasmid Expression System | Protocol Preview

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells - a 2 minute Preview of the Experimental Protocol

Francis Migneault, Frédéric Gagnon, Emmanuelle Brochiero, Yves Berthiaume, André Dagenais
Centre de Recherche du Centre hospitalier de l'Université de Montréal (CRCHUM),; Institut de Recherches Cliniques de Montréal (IRCM),; Université de Montréal, Département de médecine, Faculté de médecine;

Here, we present a tool that can be used to study the posttranscriptional modulation of a transcript in primary alveolar epithelial cells by using an inducible expression system coupled to a pipette electroporation technique.

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