Nuclear Staining Assay to Determine Mitocan Cytotoxicity | Protocol Preview

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity - a 2 minute Preview of the Experimental Protocol

Jingqi Pei, Svetlana B. Panina, Natalia V. Kirienko
Rice University, Department of BioSciences;

The protocol describes a rapid, high-throughput, reliable, inexpensive, and unbiased assay for efficiently determining cellular viability. This assay is particularly useful when cells' mitochondria have been damaged, which interferes with other assays. The assay uses automated counting of cells stained with two nuclear dyes – Hoechst 33342 and propidium iodide.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.