Virology Live #2: The Infectious Cycle

In this session we explore the infectious cycle, the complete course of events in a virus infected cell. We discuss the different parts of the infectious cycle and how to study them, including assay of infectious virus particles by plaque assay, multiplicity of infection, and the particle to pfu ratio. Physical methods for detecting viruses include hemagglutination, serological techniques, polymerase chain reaction, and high-throughput sequencing. We show how these methods were used to identify in early 2020 the pandemic coronavirus SARS-CoV-2.

Room Name: Virus

——————————— CONNECT ———————————
Twitter: @profvrr

——————————— MORE VIROLOGY ———————————

——————————— OUR OTHER PODCASTS ———————————

– Who Am I? –

I’m Vincent Racaniello, Earth’s Virology Professor.

I’m also a professor of virology at Columbia University in New York. I’ve been doing research on viruses since 1976, and teaching virology in classrooms and online since 1999. On this YouTube channel I share videos of my lectures, podcasts, and more.

New videos are uploaded every Thursday and Sunday mornings.

I do not run ads on our work as it is disruptive to learning. We depend on your support.

———————————————————————
#microbe #viruses #coronavirus #pandemic
———————————————————————

[lorriemoody]

I'm just a country girl from Actually, a retired middle school teacher. I taught mainly math and science. I'm finding your channel so informative and thought provoking. I found your channel this past year as I was hunting for reliable sources of information on Covid. Thank you so much for ALL you have been presenting. You have awakened some brain cells in this old school teacher's head and I've suggested your channel to many of my friends. Thanks again!

[Invisbke]

You make we want to go back to school. Thanks for taking the time at the best of moments.

[claudia]

Hi Dr. Racaniello. I am really enjoying your lecture, and I plan to finish the course. I'll have to watch it after you teach it, because of time constraints. Thanks so much for educating us about viruses 💐

[MrOzzyCam]

Thanks Vincent for another great lecture.

[klauswind]

Kudos….100k subs. Now, 500k or bust!!

[jballenger]

Congratulations !!!! 🥳🥳🥳🥳 🎈👉🏼👉🏽 100K Subscibers 👈🏼👈🏽🎈FAN-tastic milestone!!!!🥂🎈🥂🎈🥂

[sleepydrJ]

But in the early days of PCR people used Klenow as the pol (over and over and over), the brilliant step forward was to use taq. I remember a couple awful afternoons attempting it before taq. Total experimental failures!!

[SophyaAgain]

Just read the chat. Of course, that's the MicroTV tradition. Say hello and tell the weather. Folk get your thumbs up. That helps the channel. Cheers.

[boovan]

@Vincent Racaniello can’t find this quiz, taking class after live presentation. I found the first one is there a second one?

[sivasankar]

During TCID50 calculation If i get CPE only in 10 power -1 dilution, is it possible to calculate? or is the test valid?
Thanks

[frankblack]

lol "This is not DHTS" @1:37 but a lot more fun using P32/SDS acrylamide.

[briancase]

It's like going back to community college...if your community college were an Ivy League school. :)

[tomsteinberg]

Correction: my Livestream comment at near 1 hr 36 minutes was not right -- phosphorescence also requires incident light. The term I wanted was: bioluminescence (those sea organisms that light up at night; also, fireflies, -- e.g. also, see luciferase). A bioluminescent organism can have a fluorescent protein as an acceptor and secondary emitter, but that's not required.

[chriswilliams]

How rude. You don't need a hair cut. You're fine.