Kirby-Bauer Test time-lapse with E . coli and S. aureus

A time-lapse of the Kirby-bauer test done with E. coli and S. aureus. The Kirby-Bauer test uses Mueller-Hinton agar to test a bacterium's susceptibility to antibiotics by using discs that have antibiotics in it.
Antibiotics can work in one of two ways: they either slow down growth (bacteriostatic) or kill the bacteria (Bactericidal). A lot of antibiotics target the thick peptidoglycan layer of the cell wall in gram positive organisms, which makes gram negative bacteria more resistant to these antibiotics.
If the bacteria is susceptible to the antibiotic, it will create a zone of inhibition which is the area around the antibiotic disc where the bacteria did not grow. A larger zone indicates greater susceptibility.

Cultures were grown at approximately 37 degrees Celsius.

[serbien]

S. Aureus deserves a whole video, since MRSA and Vancomycin resistance in S. Aureus are becoming more and more common.

[takoyuuki]

Thank you so much for this! It turned out very useful for minor research for my project

[MaimunaMakamaShafii-yufk]

Please what's the diameter of zone of inhibition of tetracycline on staphylococcus aureus

[aashishsharma]

Could milk enrichment of Kirby Bauer be done? Staph aureus is beautiful in that if i'm not mistaken

[tarapigott]

Hi Kirby,
I was just wondering what sort of Penicillin did you use in this experiment for E. coli?

[sundrastinson]

I have a question? If you perform a kirby Bauer test using E. coli and test it against trimethoprim and your disc is to close to the edge of the petri disc and you are not able to measure a full diameter, only a radius toward the center of the plate-- a radius of 6 mm. How would you report the test?

[markarca]

This is essentially an antibiotic sensitivity test.

[fin]

I have staph capitis in my scalp for years, antiobitic removes it but it comes back even worse, help

[markarca]

S. aureus is a part of the normal bacterial flora of the skin.