Chemical/Laboratory Techniques: Column Chromatography

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This video was created by N.N.H.M. Eisink, PhD, and T.R. Canrinus, PhD of the Faculty of Engineering and Science of the University of Groningen as an Open Educational Resource (OER)

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[TanveerAhmad-eurq]

Hello Respected Sir.... Sir kindly make lectures on Gas chromatography and HPLC..

[salehchem]

thank you. If I used wet packing of silica gel to column (slurry),
here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.

[salehchem]

thank you so much. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me

[salehchem]

If I have solid sample containing components,
can I use any solvent to dissolve it?
Or I have to use only the same mobile phase as solvent to dissolved sample?
Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot