DNA Cloning | Part 2 - Enzymes used in Gene Cloning

Friends, In this video we discuss about uses of engymes in DNA Cloning.

Basic Info about DNA Cloning enzymes:
Two enzymes are used to produce recombinant plasmids. Restriction enzymes cut DNA at specific 4- to 8-bp sequences, often leaving self-complementary single-stranded tails (sticky ends). These enzymes are used to cut long DNA molecules into multiple restriction fragments and to cut a plasmid vector at a single site.

Hope you find this video helpful. In case you have any doubts please feel free to ask in comments. I keep checking comments and reply to them.

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Thanks for watching.

#DNA #Cloning #BaaYo

[mayurbiotech]

Is video se pata chala kitna beautiful hamara nature hai insan ne socha ki kisi disease ko jad se treat kare to nature ke pass already solution tha, iska matlab jo insan ne socha wo already existed tha bas use implement karna tha, aur har aage ki treatment bhi nature me already exist karti hi hogi ab bas wo kaam kisi ko saupa gya hai jo us direction me jake khoj nikalega

[divyasahu]

Tq mam aap pura prosese explain kr rh h
Or jyada janne ka mn kr ra h ab to....

[ammarahchaudhary]

Plz made video on DNA replication, transcription translation in eukaryotes prokaryote in full detailed for m Phil students.

[tamanaah-ug]

Thank you 🙏 ma'am 😊😊lots of love 😘

[rollnoomkarghatvilkar]

Maam Could you plz give all enzymes and their functions which is required for the cloning and recombinant DNA before my exam

[shyamchaudhary]

Mam your every video most useful far us

[pratibhatomar]

Mam your videos are very helpful for me
Mam please tell me origin of replication in plasmid please mam🙏🙏

[charaniyavandanab.]

Well understood 🤗Thank you so very much.

[reetikajaiswal]

Mam please don't teach use wrong...you saying that klenow sequence is onely performing 1 activity but it can perform more

[waliullah]

Thank u for uploading such a well explained video Lecture..😊

[amritabioclass]

Thanku so mouch mammm and very very nice video

[poriyanikunj]

Mam.. pls explain preparation of cDNA and prpblems in cDNA preparation and cloning of mam pls explain this topic

[swastikkumar]

Is there difference between Nick and Gap during polymerase activity?

[BeyondBeautyAisha]

Mam can you please provide the link of the book from which you have taken this lecture.

[Smiley]

Mam ye apki video iit jam aur gate b ke liye dekh sakte hai benefits honge yaa high level ka hai apke lecture....

[shanzaymalik]

I didn`t found DNA cloning Part 1 video on your channel

[mousumisultana]

Ma'am cell harvesting method ke upr video chahiye

[manavsingh]

Ma'am I Have A Question Jaisa Ki Apne Btaya Ki Tube m ORI DnA Mil jata Hai Incubation K bad To Ma'am Hum Y Kaise Dekht Hain Ki Y Vhi DNA Hai...Aisi Konsi Technique Use Krtr Hajn..Y Fr Instument..Plz Answer🙏🙏

[kanhaiyalalkumhar]

Ma'am ek video plant and animal cell culture pr bi banao

[jabifatma]

Ma'am..why taq polymerase don't hv proof reading activity??? Plz reply