Heat-Shock Transformation Protocol (for Bacteria)

Hey Friends,

the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.

Transformation, Conjugation and Transduction:

References:

Thanks
Henrik

Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro

[pitkamatka]

Your videos are very concise and cut right to the chase. I'm going to watch them all.

[joannapikula]

Thank you so much for explaining this so clearly and fully! Great video xx

[snehapathak]

Thanks for correct and full explanation .

[mohammedal-hammadi]

Thank you really... I hope you will make new videos soon.

[Jojochecker]

I'm happy you wrote the name of it. First I thought your talking about a body transformation of a famous film director haha

[anamilheiro]

Hello, great video! What's the ice for? Does it enhance the heat-shock or is it essential for the DNA treatment with CaCl2?

[elizaledru]

THANK YOU VERY MUCH !!! subtitles in french please : )

[bts_rollercosterlifepurplearmy]

Where is Electroporation method video ???

[shreyaskulkarni]

I want multiple likes button to this channel 🔥🔥🔥

[skippy]

Everyone here writing a lab report ngl