Creating Phylogenetic Trees Using NCBI & MEGA

[Bigtigerfanmom]

Here's a Google Drive with some examples to explore:

[sarahpopple]

Even with a more recent version of MEGA this tutorial still worked perfectly! Thank you :)

[veharaariyaratne]

This was soooo helpful for an assignment I was stuck in because MEGA kept rejecting the sequences I aligned on YOU ARE A LIFE-SAVER HONESTLY THANK YOU SO

[anamesquita]

Jesus Christ, finally someone with an understandable accent. Thanks.

[H-Rexz]

Thank you Lisa this video is easy to follow and all steps are clear.

[ndriunitedagbians]

Thank you so much madam. i was struggling a lot. but your video really helped me. i am your fan from India.

[TheDBZgamer]

ur the best....thank you..and yes i drink milk and read books...

[akinwoleadeyeye]

Thank you very much Dr.

God bless you

[MuhammadHanif-ljry]

very nice Dear Madam
I accept you as my teacher

[mushtaqal-esawy]

Really helpful, many thanks Lisa! Can I ask about the percentage or numbers that can be added to the tree image and if I can add a unit to the scale?

[MichaelWitbrock]

Of course, you or I will not have the pre-loaded phylogentic tree file with the 5 other kinds of Kluyveromyces partial ribosomal genes waiting to be added in "edit". And they are extremely hard to search for in NCBI. (the Accession numbers in the screenshot aren't very helpful. BUT the GI numbers like 31581082, 4038945 etc (at the left of the species ID in MEGA) are. So, tediously search for each of those, send it to a FASTA file, and insert each of those files into MEGA. Sigh. Also, the automated open in MEGA on download thing seems to be a Mac "feature" and super dangerous. If on another kind of computer, just insert the file in edit mode.

[june]

Good video. A few queries: what do the nos 0.001, 0.003 in the clades mean? How can I do bootstrapping? And how do I add the timescale?

[arsivbeyin]

hank you so much! This was really helpful.

[suvigyasharma]

Thanks, Lisa for the video! I found it!

[rohanmalik]

do we need to clean the sequences before running mega?

[joannalynmontemayor]

Hello Ms. Lisa, where can I possibly start trimming my allignment from the start and end region.

[miguelangelalmanzamorales]

thank you so much! This was really helpful

[sindibin]

Thank you very much, it is well infomative lecture

[bramantyowikantyoso]

I tried to do the same, but Mega opened it with txt file, not directly to allignment explorer? am i doing correct way as you did?

[sihammicrobio]

Thanks allot for this greatfull simply vedio, my question is how we can know the code of for example bacteria ? What's the step to do that?