Using multiplex IF to uncover immune cell signatures in the TME using customizable InSituPlex assays

In translational oncology research, understanding and evaluating the phenotypic profiles of cells in the tumor immune microenvironment can yield deeper understanding into the complex interactions within the immunological landscape of the tumor.

Multiplex immunofluorescence can study this interplay through rapid identification, quantification, and mapping of many cell types. Having the flexibility to look for specific biological markers and their co-localization within this environment allows researchers to better understand the mechanisms of action for compounds of interest.

Here, Scott Lawrence shows his work at the NCI using a customized 8-plex mIF panel to study markers in a multitude of cancer types and discusses the insights gained from data gathered through staining, imaging, and analysis.

Key takeaways
• Multiplex immunofluorescence is a beneficial tool in understanding and characterizing the tumor microenvironment
• InSituPlex is a ready-to-deploy, high-throughput mIF platform that is workflow-agnostic and provides whole-slide biomarker profiling
• Fast, customizable mIF panels accelerate the hypothesis-to-answer workflow and can be key in gaining insights about targets of interest tailored to specific projects