Rothera's test - for detection of ketone bodies in urine

Practical demonstration
For Medical and Dental students
Important test in abnormal constituents of urine

[sayanibasu__]

Principle- acetone and acetoacetate reacts with sodium nitroprusside in the presence of alkali to produce a purple ring at the junction.

[عامرحسين-وح]

Thankkk y i am very happy and understanding from explanation

[Bionika.]

Plz upload more test and practical plz sir it's my huge request 🙏

[INDIANPATRIOT-lszk]

Supplementary ka practical dene jaara hu aur 1 ghante pehele practical dekhra hu

[Abhishek-isvm]

Nice video but you need to mention the amount of ammonia solution added

[sandhyaarun]

Very nice vedio but quantity and concentration is not mentioned .. pls do mention

[rahulmooshahari]

Thank you so much sir. why we are giving liquid ammonia from the side of a test tube??

[sourangambarkandaj]

Purple ring at the junction between two solutions (two solutions means? Urine sample and ketone body ?)

[mujeebwani]

Why we use ammonium sulphate for saturation please reply

[aprilmanipol]

Is the ferric chloride test (Gerhardt's) was made before the urine reagent strip?

[radhakrishnanravichandran]

Why is the urine saturated with ammonium sulfate in this test?

[Dr.SouravMandal]

Good..but your saturation process was not right.

[asmaulmallick]

Sir, plese tell me how many graded of sodium nitroprusside

[subhodipghosh]

Can i use supartant urine for this test???

[dk]

What if you add them all then shake (not shaking between every step) will the violet color appear?
Please someone answer me

[naphisabetpakyntein]

In my college, we have with sodium nitroprusside crystals... But on doing the same test... It didn't work... IDK y... The college confirmed it was urine containing Ketone bodies... 😂😂😂😂