The CLIA immunoassay - How do kits work?

How do kits work?
The CLIA immunoassay is based on the principle of magnetic particles and their ability to separate the immunocomplex with a selected antigen or antibody.

The detection system uses flash chemiluminescence using acridinium ester and the intensity of the light flash is measured in relative light units (RLU).

Learn more about the CLIA method
Chemiluminescence immunoassay (CLIA) combines chemiluminescent technology with immunochemical reactions. CLIA technology involves the immobilization of an acridinium ester-labelled antigen-antibody complex on a solid base. Acridinium ester molecules have the dominant property of not losing luminescent activity even after binding to an antigen or antibody. Acridinium ester exposed to an alkaline solution together with hydrogen peroxide causes a flash of light.
The onset of light emission usually occurs within a few seconds. The reaction results in generation of light, the intensity of which is directly proportional to the amount of labelled complexes present and allows the quantification of the analyte.