Fluorescence Spectroscopy Intro (Lumina Fluorometer)

[acr]

The goal of the emission scan is to detect light emitted will be at a longer wavelength than the excitation photons. If the emission scan were to start at 480 nm, the spectrum might be dominated by scattered 490 nm excitation photons, which are not the result of fluorescence events. If NO fluorescence occurs, the ONLY photons which will be detected are the original 490 nm excitation photons.

[collegegirlchemistry]

I am literally the dumbest graduate student ever so thank you so much for this video now I know how to design my experiment

[Rungus]

if the molecule excites at 490 nm, why did you start the scanning at 500 nm? I just did a lab on this and im still confused.

[biyabi]

You mentioned that as you increase the voltage, you increase the intensity and the sensitivity. So, why you didn't use the highest voltage available? Is there a rule for what value you should use. Thank You

[soubhikdas]

You don't know how hold a cuvette