Using the Analyze Particles function in ImageJ to count fluorescent particles

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Quick tutorial for how to threshold your image to make everything binary and then how to use the Analyze Particles function for automatic counting of all circular particles. This can be used for counting fluorescent particles, cells, etc.!

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Hi, I have a question, if the size of the particles you're interested in is in the 10-20 pixel range, do you input that range as is in the size (pixel^2) window or do you square that first and input 100-200?

karimzedan
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Thank you for the video! How difficult is it to measure mean intensity rather than size? I have a three channel fluorescence image, I'd like to use the DAPI channel to isolate individual nuclei, then measure the mean intensity nucleus by nucleus in the other two channels. Is this possible?

sdsiljee
Автор

Could we differentiate the particle like metal, nonmetal, fiber etc..

NandkishorDeshmukh-ekpt