Experimental Design Best Practice for Multicolor Flow Cytometry

Presented By:
Carol Oxford - Field Applications Scientist, Thermo Fisher Scientific

Speaker Biography:
Carol Oxford is currently a Field Applications Scientist with Thermo Fisher Scientific. She has over 30 years of flow cytometry and cell sorting experience, including as director of the UC Davis Flow Cytometry Core Facility. She has worked for FlowJo, LLC and was one of the founding partners of ExCyte, a flow cytometry education company. She is passionate about all things flow.

Webinar:
Experimental Design Best Practice for Multicolor Flow Cytometry

Webinar Abstract:
Multicolor flow cytometry is a critical technique for many scientific endeavors, and a good experimental design is crucial for generating useful data. Being able to differentiate between the dyes and understanding the fluorochromes selection process is just the beginning: it’s vital to include the control controls, titrate each reagent, and to consider important concepts like spillover and spreading. In this webinar, we’ll discuss tips for ensuring a successful experiment and straightforward analysis.

Learning Objectives:

Understand the importance of titrating each reagent.
Comprehend important concepts like spillover and spreading that aid in fluorochrome choice for multicolor design.

Earn PACE Credits:

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