SnapGene Tutorial Part 5: Ligation to a TA cloning vector and digestion with restriction enzymes.

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I made this video when I was a graduate teaching assistant and Part 5 was intended for teaching supplementary for other instructors in the lab, but students may find this useful too. Part 5 focuses on how to ligate an insert to a TA-cloning vector followed by digestion with restriction enzymes.
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bro thank you for the information. it is quite helping easy to understand has I am bringer using snap gene software. Thank you again.

CHETHANANANDASANJEEVAIAH
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I have a question, how to design a primer for the DNA sequence which has HisTag at the end?

CHETHANANANDASANJEEVAIAH