DNA Quantitation Using a Spectrophotometer

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This video demonstrates how to quantitate DNA using a UV-capable SmartSpec™ spectrophotometer. Plasmid DNA is measured, showing step-by-step instructions for the instrument. Purity of the DNA is assessed using the A260/A280 ratio.

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thanks, also nice matching green buttons, gloves and tube racks: outstanding attention to detail.

RandomNooby
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Very helpful...was looking for such vedio for a year
Thanks

muzamilmanzoor
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by OD rule of DNA, 1 OD=50 ug/ml. then it should have been 0.55 ug/ml. any reason for this minor variation?

spkmtube
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I thought for DNA 1.8 is the perfect purity and for RNA it is 2. Why is it so high here?

zauberdiva